Real-Time PCR

Real-time PCR service is making available an instrument that allows the quantification of genes expression. This instrument is a LightCycler 480 (Roche Diagnostics) using 96 and 384 wells plates.

Moreover we are providing a capillary electrophoresis device for the assessment of the proteins and nucleic acids quality (Experion, Biorad).

Those open access instruments can be used after training.

The new users have to contact Christophe Piesse or one of the other referents.

Referents:

Material

  • LightCycler 480 (Roche Diagnostics)

This Q-PCR instrument allows the quantification of genes expression and genotyping. It can be used either with 96 or 384 wells plates. It is compatible with all types of chemical probes. After an appropriate training, this instrument is in open access.

  • Experion (Biorad)

The real-time PCR facility comprises an Experion that determines the samples quality.

The Experion is an automated facility for nucleic acid analysis (doc 1doc 2) that incorporates LabChip technology into an integrated system that performs all of the steps of gel-based nucleic acid electrophoresis in one compact, durable unit.