Regulation of cell polarity during epithelial-to-mesenchymal transition and cancer progression

12 décembre 2018 - 12H00

Manuel THERY, CytoMorpho Lab, Hopital Saint Louis

Lieu : Auditorium du LBD, bâtiment C, 7ème étage

Résumé : During epithelial-to-mesenchymal transition (EMT), cells lining the tissue periphery break up their cohesion to migrate within the tissue. This dramatic reorganization involves a poorly characterized reorientation of the apicobasal polarity of static epithelial cells into the front-rear polarity of migrating mesenchymal cells. To investigate the spatial coordination of intracellular reorganization with morphological changes, we monitored centrosome positioning during EMT induced either by TGFb or by expression of ZEB1. We also analyzed the centrosome position in multiple breast cancer cell lines of known invasiveness. Centrosomes were found close to cell-cell junctions in cells adopting an epithelial state and progressively moved toward the cell center as cells transited toward the mesenchymal state. We eventually analyzed the respective role of actin filaments and microtubules in this reorientation of cell polarity.

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