15 novembre 2019 - 13h30

Ludovic Jullien, ENS, Pasteur, Paris

Résumé : Living matter provides major challenges for modeling. One avenue concerns defining a level of description, which can be experimentally addressed. The question of the observables is crucial with respect to this issue. In this lecture, I will recapitulate state-of-the-art of the relevant observables, which have been made available over the years by chemists. I will more specifically focus on fluorescence imaging, which is presently (and will remain) central in the practice of biologists. In particular, I will introduce our recent developments aiming at providing access to highly multiplexed observations while overcoming several present limitations such as interference by ambient light or autofluorescence.

Recent references

1. R. Zhang, R. Chouket, A. G. Tebo, M.-A. Plamont, Z. Kelemen, L. Gissot, J.-D. Faure, A. Gautier, V. Croquette, L. Jullien, T. Le Saux, A simple imaging protocol for autofluorescence elimination and optical sectioning in fluorescence endomicroscopy, Optica, 2019, 6, 972-980; 2. R. Zhang, R. Chouket, M.-A. Plamont, Z. Kelemen, A. Espagne, A. G. Tebo, A. Gautier, L. Gissot, J.-D. Faure, L. Jullien, V. Croquette, T. Le Saux, Macroscale fluorescence imaging against autofluorescence under ambient light, Light Sci. Appl., 2018, 7, 97; 3. J. Quérard, R. Zhang, Z. Kelemen, M.-A. Plamont, X. Xie, R. Chouket, I. Roemgens, Y. Korepina, S. Albright, E. Ipendey, M. Volovitch, H. L. Sladitschek, P. Neveu, L. Gissot, A. Gautier, J.-D. Faure, V. Croquette, T. Le Saux, L. Jullien, Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations, Nat. Commun., 2017, 8, 969; 4. J. Quérard, T. Le Saux, A. Gautier, D. Alcor, V. Croquette, A. Lemarchand, C. Gosse, L. Jullien, Kinetics of reactive modules adds discriminative dimensions for selective cell imaging, ChemPhysChem, 2016, 17, 1396-1413.

Lieu : Auditorium C707

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Séminaire externe de l'IBPS