Master-2 Project 2018-2019 : Functional analysis of Shroom proteins during pronephros morphogenesis in Xenopus

Chronic kidney disease (CKD) affects 750 million people around the world. Mutation of Shroom 3  has been identified in Human and mice associated with CKD but also neural tube closure defect. Shroom3 encodes an intracellular protein located at adherent junctions where it generates contractile forces by controlling the distribution of Myosin II downstream of the PCP pathway. [...]


Stage Master : Modélisation de la dynamique temporelle des divisions des cellules précurseurs neurales au sein de l'épithélium dorsal de la drosophile. Responsable: Michel Gho

Les soies mécanosensorielles qui couvrent la partie dorsale du thorax de la drosophile sont formées au sein de l'épithélium à partir de cellules précurseurs. Ces dernières sont organisées en rangées, s’étendant sur toute la longueur, parallèles à la ligne médiane. Ces cellules précurseurs restent arrêtées environ 10 heures en phase-G2 puis entrent en division pour générer les cellules formant chaque soie sensorielle. [...]


Projet thèse équipe Breau

Marie Breau, qui vient de créer une jeune équipe affiliée au Laboratoire de Biologie du Développement à l’IBPS et au Laboratoire Jean Perrin (Physique) et Alain Trembleau (Lab. Neuroscience Paris Seine, IBPS) recherchent un(e) très bon(ne) candidat(e) à présenter au concours de l'Ecole Doctorale Complexité du Vivant. [...]


Exploring the function of an RNA methyl transferase (RMT) involved in non-coding RNA silencing pathways (mi, si &piRNA).

Three classes of small non-coding RNAs (sncRNA) emerged as a vast silencing framework in metazoan. They are involved in numerous biological processes including development, defense against pathogens and transposable element as well as chromatin dynamics. Precursors of these three types of small RNAs, namely miRNAs (microRNA)siRNAs (small interfering RNA) and piRNAs (piwi interacting RNA) are all transcribed by RNA polymerase II. After distinct biogenesis processes, si, pi and miRNAs are all loaded inside Argonaute proteins and guide them to target RNAs with sequence complementarity. The post-transcriptional repression that will take place using these ribonucleoprotein complexes (RNA-Induced Silencing Complexes, RISC) can operate through endonucleolytic cleavage or translational repression of the target RNA. Argonaute proteins loaded with si or piRNA are also involved in transcriptional repression by recruiting, at nascent RNAs, complexes that are able to modify DNA or histone proteins. These varieties of functional and mechanistic outputs explain why misregulation of RNA silencing pathways has been correlated to the emergence of cancer, neurodegenerative diseases and infertility troubles. [...]